Immunolocalization of chitin synthases in the phytopathogenic dimorphic fungusUstilago maydis
نویسندگان
چکیده
منابع مشابه
Proteomic analysis of dimorphic transition in the phytopathogenic fungus Ustilago maydis.
In the corn smut fungus Ustilago maydis, the dimorphic transition from budding to filamentous growth is intrinsically associated with the switch from a saprophytic to a pathogenic lifestyle. Both pathogenicity and filament formation are triggered by a heterodimeric homeodomain transcription factor encoded by the b mating type locus. Here, we present a reference map of the proteome of this dimor...
متن کاملPheromone-induced G2 arrest in the phytopathogenic fungus Ustilago maydis.
In the corn smut fungus Ustilago maydis, pathogenic development is initiated when two compatible haploid cells fuse and form the infectious dikaryon. Mating is dependent on pheromone recognition by compatible cells. In this report, we set out to evaluate the relationship between the cell cycle and the pheromone response in U. maydis. To achieve this, we designed a haploid pheromone-responsive s...
متن کاملPolar localizing class V myosin chitin synthases are essential during early plant infection in the plant pathogenic fungus Ustilago maydis.
Fungal chitin synthases (CHSs) form fibers of the cell wall and are crucial for substrate invasion and pathogenicity. Filamentous fungi contain up to 10 CHSs, which might reflect redundant functions or the complex biology of these fungi. Here, we investigate the complete repertoire of eight CHSs in the dimorphic plant pathogen Ustilago maydis. We demonstrate that all CHSs are expressed in yeast...
متن کاملGene transfer system for the phytopathogenic fungus Ustilago maydis.
A selectable marker for transformation was constructed by transcriptional fusion of a Ustilago maydis heat shock gene promoter with the hygromycin B phosphotransferase gene of Escherichia coli. U. maydis was transformed to hygromycin B resistance by polyethylene glycol-induced fusion of spheroplasts following exposure to plasmid DNA that carried the marker gene. Transformation frequencies of 50...
متن کاملSpecific b alleles matter in phytopathogenic Ustilago maydis
SPECIFIC b ALLELES MATTER IN PHYTOPATHOGENIC USTILAGO MAYDIS Kavita Burman Aulakh
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ژورنال
عنوان ژورنال: FEMS Yeast Research
سال: 2006
ISSN: 1567-1356,1567-1364
DOI: 10.1111/j.1567-1364.2006.00133.x